Tg mbp:mcherry-caax
WebOur analyses of myelination using Tg(mbp:EGFP-CAAX) indicated that despite the extensive myelination of cell bodies, ... (A and B) Confocal images of single oligodendrocytes labeled mosaically using mbp:mCherry-CAAX in wildtype (A) and caspr sa12772 mutants at 5 dpf. Scale bar, 10 μm. Web22 Oct 2016 · To visualize myelination in vivo, we used the plasmid (mbp-EGFP-CAAX) to label single mature oligodendrocytes in the ventral spinal cord, and observed a roughly 30 % increase in the myelin sheath number of individual oligodendrocytes in PTZ-treated animals (10.12 ± 0.56 control vs. 13.11 ± 0.54 PTZ) (Fig. 1 c, d).
Tg mbp:mcherry-caax
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Web5 May 2016 · The transgenic reporter Tg(mbp:mCherry-CAAX) was generated as part of this investigation. Generation of Fluorescent Fusion Proteins to Visualize Myelination along Single Axons. Full-length cntn1a cDNA was amplified by RT-PCR from 3 dpf total RNA using primers 1 (EcoRI-Kozak-cntn1a-F, ... WebTg(mpeg1.1:mVenus-CAAX) Summary; Plasmid Map; Genomic Features; Transgenics; Sequence Information; Other Construct Pages; Citations; Transgenic Construct. …
Web5 Apr 2024 · We previously developed expression plasmids encoding GFP-binding peptide (or nanobody) fused to APEX, which allows EM localisation of GFP-tagged proteins in vivo. Here, we have generated conditionally stable GFP- and … WebPlasmid PhyB-mCherry-CAAX from Dr. Chao Tang's lab contains the insert PhyB and is published in Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub …
WebThat Utilize Tg(mpeg1.1:mCherry-CAAX) Construct . Genomic Feature Affected Genomic Regions sh378Tg: Transgenics . That Utilize Tg(mpeg1.1:mCherry-CAAX) Construct . Fish … WebThat Utilize Tg(mpeg1:mCherry-CAAX) Construct . Fish Affected Genomic Regions Phenotype Gene Expression; gl26Tg: zbtb11 gl11/gl11; gl26Tg: zbtb11; Fig. 3 from …
Web11 Dec 2024 · The following transgenic zebrafish lines were used: tg(sox10:mRFP) vu234 (ref. 21, kindly provided by D. Lyons, Edinburgh) and two lines generated in-house, …
Web50 rows · Tg (mbp:EGFP-CAAX) ID ZDB-TGCONSTRCT-120103-3 Name Tg (mbp:EGFP-CAAX) Previous Names None Type engineered_plasmid Regulatory Regions mbpa ( 1) … roman soldier breastplateWebengineered_region ( SO:0000804) plasmid ( SO:0000155) Children: promoter_trap_construct ( SO:0001478) engineered_episome ( SO:0000779) enhancer_trap_construct ( SO:0001479) gene_trap_construct ( … roman soldier head pewter spoons antiqueWebpEGFP-C1 (Search Vector Database) Backbone manufacturer Clontech Backbone size w/o insert (bp) 4700 Total vector size (bp) 4731 Vector type Mammalian Expression … roman society and political structureWebFor your Materials & Methods section: mCherry-CaaX (Hras) was a gift from Rob Parton (Addgene plasmid # 108886 ; http://n2t.net/addgene:108886 ; RRID:Addgene_108886) For … roman soldier headWeb24 Jun 2013 · In order to test this directly, we analyzed individual axons in wild-type and Tg (mbp:ca-Fyn) animals and found that individual myelinated axons in Tg (mbp:ca-Fyn) were covered by about 69% more myelin along their length compared to controls by 5 dpf (wild-type 35% ± 20%, 13 axons in nine animals; Tg (mbp:ca-Fyn) 59% ± 25, ten axons in ten … roman soldier helmet party cityhttp://sequenceontology.org/browser/current_svn/term/SO:0000637 roman soldier helmet miniatureroman soldier kid costume