Phgwfs7.0
WebSep 14, 2011 · GATEWAY ™-compatible destination vectors (Karimi et al., 2002) for protein subcellular localization (pH7FWG2,0), promoter analysis (pHGWFS7,0), overexpression (pH7WG2D,1) and hairpin RNA interference (pH7GWIWG2(I),0) were purchased from the Functional Genomics Division, VIB-Ghent University (Ghent, Belgium). The LR reaction was … WebThe destination vector is pHGWFS7,0 (13 kb). The donor vector is pDONR221. I attached reference protocols for single-step BP/LR gateway. Any comment and advice will be really …
Phgwfs7.0
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Websubsequent analysis Running the decideTests function using the global method from COMM 2024 at Simon Fraser University WebVIB-UGent Center for Plant Systems Biology. Gateway Vectors. Collection. pKGWFS7.
WebJun 17, 2024 · The letters (a, b, c) indicate statistically significantly different values (P < 0.05), determined using Tukey’s HSD test (b is significantly different from a, c is significantly different from b). ... (Invitrogen) recombination, then … Web... pHGWFS7 for promoter-gus constructs ( Fig. 1) : This vector with binary pPZP200 backbone ( Hajdukiewicz et al. 1994) contains CmR-ccdB selection flanked by attR1 and …
WebNdeI for a single cut fragment, L7:1kb + DNA ladder, L1-L6: Digested samples, L2: Positive sample with true single cut. 1E: Plasmid isolation from pHGWFS7.0_Adh vector. WebOct 1, 2014 · A sequence of approximately 2kb preceding the translation initiation site of the OsAPx1 gene was isolated, cloned into pENTR vector and recombined in pHGWFS7 vector, whichallows the fusion of the promoter sequence with two report genes, Gfp and Gus, and confers resistence to hygromycin.The construction was named pPROM1. The …
WebMay 27, 2014 · Sterols have important functions in membranes and signaling. Plant sterols are synthesized via the isoprenoid pathway by cyclization of 2,3-oxidosqualene to …
Webwere sterilized with 0.3% Sodium Hypochlorite and pre-germinated on 0.9% bactoagar plates at 30 C. Seven day old seedlings were transferred to sterilized sand and grown for two weeks without fertilizer. Plantlets were then transferred to liquid ½ Hoagland containing 25 M phosphate for another week before treating them either with GSE, holcombe v united statesWebEnter your name and contact information. To better ensure the security of donor accounts, we need to prove you are a human. We apologize for the inconvenience. Your donation is … hud label informationWebOct 20, 2016 · The vector pH35 was constructed from Gateway promoter-reporter vector pHGWFS7, which contained a GFP-GUS fusion for plant expression, hygromycin resistance gene ( HygR) for plant transformation and spectinomycin/streptomycin resistance (Sm/SpR) for bacterial transformation (Karami et al. 2009 ). hud landlord portal sign in new orleansWebJan 5, 2024 · Finally, MtCIR1 recombined with pHGWFS7.0 (Supplemental Fig. S3). After sequence confirmation, the terminal construct p MtCIR1 -GUS was transformed into Arabidopsis using Agrobacterium tumefaciens (strain EHA105)-mediated floral dip method (Clough and Bent 1998 ). hud lake county ohioWebwith the pHGWFS7 vector by using LR clonase. The resulting construct was transferred to Arabidopsis (Col-0) by Agrobacterium-mediated transformation. Primary transformants … hud land foreclosuresWebJan 5, 2024 · Finally, MtCIR1 recombined with pHGWFS7.0 (Supplemental Fig. S3). After sequence confirmation, the terminal construct p MtCIR1 -GUS was transformed into … holcomb extends emergencyWebThe destination vector (pHGWFS7.0) used here is efficient for promoter expression analysis (Karimi et al. 2002). Site specific recombination properties of Gateway system allowed recombination of the Adh promoter from the pENTR_Adh to … holcombe wasp exterminator