Immobilized template assays
Witryna2 maj 2013 · In this paper, we describe a peptide library designed by computational modelling and the selection of two peptide sequences showing affinity towards the mycotoxin, ochratoxin A (OTA). A virtual library of 20 natural amino acids was used as building blocks to design a short peptide library against ochratoxin A template using … Witryna9 paź 2007 · Immobilized template assays were performed by incubating nuclear extract with the indicated templates for 40 min with or without Gal4-AH (A, 6 pmol), …
Immobilized template assays
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Witryna8 lip 2011 · Immobilized Template Assays. Biotinylated DNA fragments from the plasmids pG5MLT or pREx4MLT, which contain tandemly repeated GAL4- or HNF4α-responsive elements, respectively, were generated and bound to Dynabeads (Dynal). Recruitment assays were performed essentially as described (Takahashi et al., … WitrynaHere we employed immobilized template assays to correlate transcriptional activation with mediator and TFIID recruitment. In reactions reconstituted with purified proteins, we found that activator, TFIID, and mediator engage in reciprocal cooperative interactions to form a complex on promoter DNA.
Witryna7 kwi 2024 · Plasmonic nanoprobes consisting of gold nanoparticles (AuNPs) and biomolecules are used due to their LSPR properties. In 2024, Kim et al. proposed a sensitive sandwich assay to detect the surface antigen of the hepatitis B virus (HBsAg). They used a glass substrate fabricated with AuNPs, and the antibody was … WitrynaImmobilized Template Assay. Cite this entry as: (2013) Immobilized Template System. In: Dubitzky W., Wolkenhauer O., Cho KH., Yokota H. (eds) Encyclopedia of …
Witryna6 paź 2024 · For the immobilized template assay, the same procedure was followed except for collecting the pellet and supernatant fractions at the desired time points. To map TOP2B, indicated restriction enzymes were added to the reactions at the appointed time points and allowed to sit for 15 min at 30°C. The pellet fraction was dissolved in … Witryna1 sty 2012 · The immobilized chromatin template assays were performed essentially as described previously (Lin and Carey 2012) with some modifications to the protocol. …
Witryna1 cze 2003 · Immobilized template assays were performed using beads alone or beads carrying a tRNA Leu gene fragment (McLaren and Goddard, 1986) and 200 μg of …
Witryna27 lip 2024 · The active Bro immobilized on GNPs (NanoBro) remained stable under storage and gained thermal stability consistent with a thermophilic enzyme. Proteolytic assays were performed on type I collagen membranes using fluorescence spectroscopy of O-phthaldialdehyde (OPA), changes in the membrane superficial structure, and … leecher for cracking accountsWitryna25 paź 2010 · To identify the specific histone targets of acetylation by p300, we used a chromatin-based immobilized template assay comprised of the model human T-cell … leecher githubWitrynaNational Center for Biotechnology Information how to explain a layoff in an interviewWitryna10 cze 2008 · The immobilized template assays were performed as shown in Fig. 1C; however, the nuclear extract was preincubated with Lys-CoA or curcumin, selective inhibitors of CBP/p300 acetyltransferase activity (15, 16). Each inhibitor blocks the acetyltransferase activity of CBP/p300 by a distinct mechanism. how to explain a late delivery to a customerWitryna25 paź 2010 · We utilized an in vitro immobilized template assay to investigate activator and Ac-CoA-dependent disassembly of nucleosomes from a native promoter. We compared chromatin templates composed of a mixture of wild-type histones and histones carrying lysine to arginine (K → R) N-terminal tail mutations. leecher for crackingWitryna27 kwi 2024 · Immobilized template recruitment assays. The ML promoter region of the in vitro transcription assay template was amplified using 5’-biotinylated primers. The resulting biotinylated product was bound to Dynabeads M-280 Streptavidin (Invitrogen -Thermo Fisher Scientific, Carlsbad, CA, USA; cat no: 11205D). Briefly, the beads … lee cherngWitryna15 sty 2000 · All of the immobilized DNA templates containing appropriate regulatory regions were fully competent for transcription and translation reactions and several could be re-used in serial reactions. ... Assays utilizing post-PCR immobilized DNA have been developed for a myriad of applications, including: the detection of single or multiple ... how to explain a lesson plan