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Cryaa promoter

WebJun 5, 2015 · CRYAA transcript and protein levels are both downregulated, and the CpG island in the CRYAA promoter is hypermethylated in human age-related cataract (Zhou et al., Reference Zhou, Luo, Liu, Fan and Lu 2012). We performed promoter methylation analysis of six genes, including TRAM1, CRYAA, HSF4, VEGFA, GJA3, and FTL. … WebPlasmid pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus from Dr. Didier Stainier's lab contains the inserts Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA and Zebrafish cryaa …

(PDF) Polymorphism rs7278468 is associated with Age-related …

WebMar 17, 2016 · The CRYAA promoter has transcriptional activity in HLE cells: compared with the vector, the luciferase activity was more than 10 times higher in cells transfected … WebOne of the major challenges to widespread application of Cre/loxP technology in zebrafish is comparatively small numbers of established tissue-specific Cre or CreERT2 lines. We used Tol2-mediated transgenesis to generate Tg(CrymCherry;-1.9mylz2:CreERT2) which provides an inducible CreERT2 source driven by muscle-specific mylz2 promoter. braveheart muay thai https://corcovery.com

(PDF) Polymorphism rs7278468 is associated with Age

WebFeb 22, 2024 · 66. ]. The fact that both GJA8 and CRYAA function to stimulate visual development and are required during retinal development in mice and humans suggests that GJA8 and CRYAA are key genes for the promotion of retinal development and alleviation of the symptoms of visual impairments in mammals. WebJun 20, 2016 · A Cryaa promoter-tdTomato plasmid was gifted by T. Xu (Yale) [6]. The Cryaa promoter-tdTo-mato cassette was released by restriction with MluI and AflII and ligated in compatible sites. WebAug 9, 2016 · The evidence presented suggests that the methylation of the CpG sites of the CRYAA promotor directly affect Sp1 binding, leading to down expression of CRYAA in … braveheart musical

Addgene: Zebrafish Plasmid Collection

Category:CRYAB Gene - GeneCards CRYAB Protein CRYAB Antibody

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Cryaa promoter

Epigenetic Regulation of αA-crystallin in High Myopia-Induced …

WebAug 18, 2024 · Our study aimed to conduct a meta-analysis investigating the association between polymorphisms in CRYAA and susceptibility to cataracts. Methods: The … WebDec 21, 2024 · VEGF is a key factor in neuropathology, with studies showing that single nucleotide polymorphism in the VEGF promoter region is associated with a risk of AD and vascular dementia . ... μm in size, displaced the basolateral RPE membrane and were rich in neutral lipids as well as drusen components ApoE, CryaA/CryaB and TIMP-3.

Cryaa promoter

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WebMar 21, 2024 · CRYAA (Crystallin Alpha A) is a Protein Coding gene. Diseases associated with CRYAA include Cataract 9, Multiple Types and Cataract 24. Among its related … WebPromoter SP6 and CMV Availability Industry, Academic Institutions, and Nonprofits pE-SUMO CRYAA Plasmid #80756 Purpose Expression of human alpha A-crystallin in E. …

WebMar 17, 2016 · (a) The CRYAA promoter containing either the T-C-G or C-G-T haplotype was transfected into HLE cells and Luciferase activity was measured 72 hours after … WebDec 3, 2013 · CpG islands in lens epithelial CRYAA promoter in the HMC NC5-6 Group exhibited the highest methylation of all the groups, but no statistically significant differences were evident between the HMC ...

WebPolymorphisms in alpha A crystallin (CRYAA) gene have been implicated in susceptibility to cataracts, but some published studies have reported inconclusive results. Our study … WebThe study demonstrates that residues 70-88 in CRYAA act as a primary substrate binding site and account for the bulk of the total chaperone activity. The T allele of rs7278468 in the crystallin alpha A promoter is associated with age-related cataract through increasing binding of KLF-10 and thus decreasing crystallin alpha A transcription.

WebDec 30, 2015 · Herein, we identified and characterized a novel FGF2-responsive region in the c-Maf promoter (-272/-70, FRE). Both c-Maf and Cryaa regulatory regions contain arrays of AP-1 and Ets-binding sites.

WebThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. braveheart murron actressWebFigure Legend Snippet: A. Schematic representation of elements in the Tol2-based transposon vector driving the expression of oncogenic GNAQ Q209P under the control of … braveheart musical scoreWebMar 28, 2024 · A segment of cryaa-EGFP (cryaa: α-crystallin A) was reversely inserted into the plasmid as a screening marker, so that the ocular EGFP expression enabled us to visually select the transgenic fish with the genomic incorporation. The promoter cryaa, containing a 731-bp 5′-upstream sequence of the gene, was amplified from the zebrafish … braveheart music composerWebJun 20, 2016 · A Cryaa promoter-tdTomato plasmid was gifted by T. Xu (Yale) . The Cryaa promoter-tdTomato cassette was released by restriction with MluI and AflII and ligated in compatible sites between 5´and 3´ piggyBac ITR´s, resulting in pTTCryTom (cryTom) plasmid consisting of the alphaA-crystallin promoter, tdTomato cDNA and a SV40 poly … braveheart music byWebAug 9, 2016 · CpG methylation of CRYAA promoter decreases the DNA-binding capacity of transcription factor Sp1 EMSA was performed to determine whether the methylation of … braveheart music remixWebDec 3, 2013 · DNA methylation was analyzed by pyrosequencing the CpG islands in CRYAA promoter. CRYAA expression was compared among high-myopic eyes with … braveheart music mixWebMar 17, 2016 · A 1111 bp region of the human CRYAA proximal promoter was amplified using CRYAA-Promoter-F and CRYAA-Promoter-R as described above. The PCR product was cloned into the PCR 2.1-TOPO (Invitrogen ... braveheart music youtube