WebJun 5, 2015 · CRYAA transcript and protein levels are both downregulated, and the CpG island in the CRYAA promoter is hypermethylated in human age-related cataract (Zhou et al., Reference Zhou, Luo, Liu, Fan and Lu 2012). We performed promoter methylation analysis of six genes, including TRAM1, CRYAA, HSF4, VEGFA, GJA3, and FTL. … WebPlasmid pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus from Dr. Didier Stainier's lab contains the inserts Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA and Zebrafish cryaa …
(PDF) Polymorphism rs7278468 is associated with Age-related …
WebMar 17, 2016 · The CRYAA promoter has transcriptional activity in HLE cells: compared with the vector, the luciferase activity was more than 10 times higher in cells transfected … WebOne of the major challenges to widespread application of Cre/loxP technology in zebrafish is comparatively small numbers of established tissue-specific Cre or CreERT2 lines. We used Tol2-mediated transgenesis to generate Tg(CrymCherry;-1.9mylz2:CreERT2) which provides an inducible CreERT2 source driven by muscle-specific mylz2 promoter. braveheart muay thai
(PDF) Polymorphism rs7278468 is associated with Age
WebFeb 22, 2024 · 66. ]. The fact that both GJA8 and CRYAA function to stimulate visual development and are required during retinal development in mice and humans suggests that GJA8 and CRYAA are key genes for the promotion of retinal development and alleviation of the symptoms of visual impairments in mammals. WebJun 20, 2016 · A Cryaa promoter-tdTomato plasmid was gifted by T. Xu (Yale) [6]. The Cryaa promoter-tdTo-mato cassette was released by restriction with MluI and AflII and ligated in compatible sites. WebAug 9, 2016 · The evidence presented suggests that the methylation of the CpG sites of the CRYAA promotor directly affect Sp1 binding, leading to down expression of CRYAA in … braveheart musical