WebJan 5, 2024 · A detailed protocol is provided here for the optical clearing of plant tissues using clearing reagents, ClearSee (CS) or ClearSeeAlpha (CSA). The preparation of cleared plant tissues involves three steps: fixation, washing, and clearing. Fixation is a crucial step in maintaining the cellular structures and intracellular stability of fluorescent ... WebJun 3, 2024 · Sections were stained in a solution of 0.2% basic fuchsin dissolved in ClearSee, following a protocol described by Ursache, et al. 38 except that the incubation time was reduced to 20 min, washed ...
ePro-ClearSee: a simple immunohistochemical method …
WebClearSee™ is clearing reagent for the plant, possible to make transparent the entire plants in 3 steps (1. fixed 2. washing 3. transparency). Using this reagent enables fluorescence observation up to inside of sample without cutting by removing the chlorophyll. Use of ClearSee™ allows for transparency while holding fluorescence of ... WebJan 20, 2024 · Tissue clearing methods combined with confocal microscopy have been widely used for studying developmental biology. In plants, ClearSee is a reliable clearing method that is applicable to a wide range … smadav pro registration name and key 2022
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WebDec 1, 2015 · ClearSee rapidly diminishes chlorophyll autofluorescence while maintaining fluorescent protein stability. By adjusting the refractive index mismatch, … WebJan 20, 2024 · Here, we describe a ClearSee-based clearing protocol which is suitable for obtaining 3D images of Arabidopsis thaliana embryos. The method consists of embryo dissection, fixation, washing, clearing, and cell wall staining and enables high-quality 3D imaging of embryo morphology and expression of fluorescent reporters with the cellular ... WebSep 14, 2024 · Tissue clearing methods eliminate the need for sectioning, thereby helping better understand the 3D organization of tissues and organs. In the past fifteen years, clearing methods have been developed to preserve endogenous fluorescent protein tags. Some of these methods (ClearSee, TDE, PEA-Clarity, etc.) were adapted to clear … smadav pro registration name and key 2021