Bzr1 chip
WebAug 13, 2024 · (E, F) ChIP-qPCR assay of BZR1 binding to the promoters of FER2 and FER3 genes in control and heat stress conditions. To make sure the BZR1 binding was controlled by BR signaling, ChIP-qPCR … WebJan 9, 2014 · To investigate the possible link between PIF4, IBH1, and BZR1, we conducted a ChIP analysis followed by sequencing (ChIP-Seq) on the IBH1OE line and a control line p35S::GFP. Antibodies against GFP in the GFP-tagged IBH1 were used for immunoprecipitation of the endogenous protein–DNA complex, and the obtained DNA …
Bzr1 chip
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WebThe gain-of-function bes1-D and bzr1-D mutants exhibit consti-tutive BR responses, and the bes1-D and bzr1-D proteins show significantly increased stability compared with the wild-type BES1 and BZR1, respectively (He et al., 2002; Yin et al., 2002), indicating that the stability of BES1/BZR1 is critical for their function. WebChromatin immunoprecipitation (ChIP) is a powerful method for studying TF-DNA interactions in vivo. To identify all binding sites of a TF in the genome, the DNA obtained …
WebSep 14, 2007 · BZR1 S130/134A, BZR1 S173A, and BZR1 T177A mutations conferred increased BR responses in transgenic plants. In particular, BZR1 S173A and BZR1 T177A, but not wild-type BZR1, ... ChIP was performed as described (Gendrel et al., 2002) with a minor modification. Two-week-old seedlings (1 g) of transgenic and wild-type plants (Col … WebMar 4, 2024 · EMSA and ChIP verified that BZR1 binds directly to the cis-element present in the PR5 promoter and a pull-down assay showed that TGAs, SA transcription factors upstream of PR genes, interact with BZR1 at the protein level. Crude enzyme assays demonstrated that BR C-6 oxidase activity, a CYP85A1 function, greatly increased …
WebThe Township of Fawn Creek is located in Montgomery County, Kansas, United States. The place is catalogued as Civil by the U.S. Board on Geographic Names and its elevation … WebMar 8, 2024 · Chromatin immunoprecipitation (ChIP)-qPCR analysis indicated that BZR1 bound to the P4 fragment of the P BRC1. Dual-luciferase assay showed that the activity of P BRC1 was inhibited by 67% as a result of BZR1 binding ( Fig. 2 G ), whereas a mutation of the E-box in the P4 fragment abolished the regulatory effects of BZR1 on BRC1 without ...
WebBRASSINAZOLE RESISTANT 1 (BZR1) is one of important transcription factors that regulate organ size in BR signal pathway in Arabidopsis. The function of BZR1 on organ size is well characterized in Arabidopsis, but poorly understood in maize (Zea mays). ... ChIP and RNA-seq analysis showed ZmBZR1 can directly bind to the promoter region of …
WebApr 26, 2012 · The core sequence was deposited in BZR1 gene. ChIP data revealed that LIC bound to the BZR1 promoter in various regions, such as, a, c, e, f, g and j, which were upregulated by BR treatment in the WT … prime materials recoveryWebMar 14, 2024 · Similarly, ChIP-qPCR analysis showed that H 2 O 2 did not have a significant effect on BZR1 DNA-binding capacity in plants (Supplementary Fig. 8d), suggesting that H 2 O 2 does not affect DNA ... play listenerWebJan 10, 2024 · EMSA and ChIP showed that BZR1 directly bound to the promoter of ABI5. This binding suppressed the expression of ABI5, which in turn enhanced the expression of SHB1, MINI3, and IKU2 to increase the seed size. BZR1 also bound to the promoter of SHB1 and IKU2, ... playlist el gran showmanWebNov 10, 2024 · Based on the chromatin immunoprecipitation microarray (ChIP-chip) analysis of BZR1, it was found that almost 1/3 of the members of AP2/ERF family are BR-regulated BZR1 target (BRBT) genes . Many … playliste br heimatWebDec 3, 2024 · In silico analysis showed that the promoters PILS2, PILS3, and PILS5 are direct targets of BZR1 and BES1 (BZR2) and the binding of BZR1 to PILS2 and PILS5 promoter was confirmed by chromatin immunoprecipitation (ChIP)-sequencing (Sun et al., 2024). Upon BL application, the expression of GFP and GUS fused to the promoter of … primemathWebIdentification of BZR1 direct target genes using ChIP-chip Source publication Integration of Brassinosteroid Signal Transduction with the Transcription Network for Plant Growth Regulation in ... prime materials associates incWebJul 22, 2012 · Among the six PRE members, PRE1, PRE5 and PRE6 were identified as PIF4 targets in the ChIP-seq analysis (Supplementary Fig. S6a), and confirmed by ChIP–qPCR to be direct targets of both BZR1 … prime materials recovery inc