Bca protein assay kit millipore
WebApr 11, 2024 · A BCA Protein assay kit (ZHHC Biotech Inc., Shaanxi, China) was used to determine the protein concentration according to the manufacturer’s instructions. The … WebMeasuring protein content using BCA assay. b. Measuring DNA content using Picogreen staining. However, these two assays gave me different result. I'm wondering: 1. Which assay is better...
Bca protein assay kit millipore
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WebMar 17, 2024 · Total protein concentration was measured using the BCA method (Pierce™ BCA Protein Assay Kit; Thermo Fisher Scientific, Warsaw, Poland). ... (Millipore, Germany). 4.9. Statistical Analysis. The obtained data were analyzed with the two-tailed Student’s t-test or ANOVA with the post hoc Bonferroni correction. The correlation … WebProtein concentration should be determined by BCA assay with BCA Protein Assay kit. Follow the instructions from Thermo Fisher Scientific. 2. Adjust the volume of 200 μg of protein to 80 μL (2.5 μg/μL) with Solution 4 (1% SDC in 20 mM HEPES, pH 7.4). The amount of protein is dependent on the aim of experiment or type of sample.
WebThis assay can beused to quantify protein concentration with awide variety of samples and can be performedin minutes.The Novagen ® BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 µg/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size …
WebThe BCA Protein Assay combines the protein-induced biuret reaction (see above) with the highly sensitive and selective colorimetric detection of the resulting cuprous cation (Cu1+) by bicinchoninic acid (BCA). Thus, two steps are involved. First is the biuret reaction, whose faint blue color results from the reduction of cupric ion to cuprous ion. WebProduct Specs Item Nuclei Isolation Kit: Nuclei PURE Prep Company MilliporeSigma Price Pricing Info Supplier Page View Company Product Page Catalog Number NUC201 Quantity 1KT Applications cell analysis MilliporeSigma 400 Summit Drive Burlington, Massachusetts 01803 United States Phone: (800)-645-5476 (800)-645-5439 Company Profile
WebAug 8, 2024 · Renal tissues were isolated from mice immediately after sacrifice, homogenized in 1:9 (w/v) normal saline with glass homogenizer for 1 min and centrifuged at 12, 000 rpm for 20 min. Protein concentration was determined by BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as a standard.
WebHowever, please note that due to variations in vendor agreements, the final pricing of special orders over $350.00 from the Enzyme Freezer Program may exceed catalog pricing. Please inquire about pricing prior to placing your order. Call Chrissy Battaglia at (970) 491-3459 for more information. Inside CSU's Life Science Stockroom terry cotton hooded bathrobeWebMillipore (1) Sigma-Aldrich (2) Quality Level. Available for Sale. United States Globally. pierce bca protein assay kit. Applied Filters: Keyword:'pierce bca protein assay kit' Showing 1-3 of 3 results for "pierce bca protein assay kit" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. triglyceride levels canada chartsWebMillipore bca protein assay kit Bca Protein Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO … terry cotton bathrobe luxuryWebApr 11, 2024 · A BCA Protein assay kit (ZHHC Biotech Inc., Shaanxi, China) was used to determine the protein concentration according to the manufacturer’s instructions. The lysates were separated by SDS–polyacrylamide gel electrophoresis and blotted onto polyvinylidene difluoride membranes. triglyceride lowering foods list pdfWeb$197.40 / Each Description EMD Millipore Novagen™ BCA Protein Assay Kit is based on biuret reaction, which is the reduction of Cu2+ to Cu+ by proteins in alkaline solution. … triglyceride lymphomaWebThe Novagen® BCA Protein Assay Kit can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration. Kit … terry cotton shortsWebMar 5, 2012 · Beta-Secretase activity assay: Activity of beta-secretase was evaluated using a FRET-based assay system (EMD Millipore). Increasing concentrations of brain tissue lysate were added to wells containing a fluorogenic peptide substrate and incubated for 1 … terry cotton kids bathrobe